MAHALLIY DORIVOR O’SIMLIKLAR ASOSIDA TABIIY ANTIBAKTERIAL FITOPREPARATLAR YARATISH VA ULARNING FARMAKOLOGIK SAMARADORLIGINI BAHOLASH
DOI:
https://doi.org/10.5281/Keywords:
dorivor o’simliklar, fitopreparat, antibakterial faollik, Allium sativum, Nigella sativa, Ocimum basilicum, Mentha piperita, disk-diffuziya, MIC, tabiiy antibioticAbstract
Ushbu maqolada O’zbekiston hududida keng tarqalgan dorivor o’simliklar asosida tabiiy antibakterial fitopreparatlar yaratish istiqbollari hamda ularning farmakologik samaradorligini baholash masalalari yoritilgan. Tadqiqot obyekti sifatida Allium sativum L. (sarimsoq), Nigella sativa L. (qora zira), Ocimum basilicum L. (rayhon) va Mentha piperita L. (yalpiz) tanlab olindi. O’simlik xomashyolaridan suvli va etanol ekstraktlari olinib, ularning antibakterial faolligi in vitro sharoitda disk-diffuziya usuli hamda minimal ingibitsiya konsentratsiyasi (MIC) ko’rsatkichlari asosida baholandi. Tadqiqotda test-mikroorganizmlar sifatida Escherichia coli va Staphylococcus aureus bakteriya shtammlaridan foydalanildi. Olingan natijalar dorivor o’simlik ekstraktlarining turli darajada antibakterial faollikka ega ekanligini ko’rsatdi. Sarimsoq va qora zira ekstraktlarida antibakterial ta’sir nisbatan yuqori bo’lib, bu ularning tarkibidagi biologik faol oltingugurtli birikmalar, allitsin, timoxinon va boshqa fenolik komponentlar bilan izohlanadi. Rayhon va yalpiz ekstraktlari ham bakteriyalar o’sishini susaytiruvchi ta’sir ko’rsatdi. O’simlik ekstraktlarini kombinatsiyalangan holda qo’llash ayrim hollarda alohida ekstraktlarga nisbatan yuqoriroq samaradorlik berishi kuzatildi. Tadqiqot natijalari mahalliy dorivor o’simliklardan tabiiy antibakterial vositalar va istiqbolli fitopreparatlar yaratishda foydalanish mumkinligini ko’rsatadi.
References
1.Hossain M.S., va boshq. A review of ethnobotany, phytochemistry, antimicrobial pharmacology and toxicology of Nigella sativa L. // Biomedicine & Pharmacotherapy. – 2021. – Vol. 143. – Article 112182.
2.Abbas M., va boshq. Antimicrobial Properties and Therapeutic Potential of Bioactive Compounds in Nigella sativa: A Review // Molecules. – 2024. – Vol. 29, No. 20. – Article 4914. – DOI: 10.3390/molecules29204914.
3.Chatterjee G., Saha A.K., Khurshid S., Saha A. A Comprehensive Review of the Antioxidant, Antimicrobial, and Therapeutic Efficacies of Black Cumin (Nigella sativa L.) Seed Oil and Its Thymoquinone // Journal of Medicinal Food. – 2025. – Vol. 28, No. 4. – P. 325–339. – DOI: 10.1089/jmf.2024.k.0149.
4.Forouzanfar F., Fazly Bazzaz B.S., Hosseinzadeh H. Black cumin (Nigella sativa) and its constituent (thymoquinone): a review on antimicrobial effects // Iranian Journal of Basic Medical Sciences. – 2014. – Vol. 17, No. 12. – P. 929–938.
5.Chaieb K., va boshq. Antibacterial activity of thymoquinone, an active principle of Nigella sativa, and its potency to prevent bacterial biofilm formation // BMC Complementary and Alternative Medicine. – 2011. – Vol. 11. – Article 29. – DOI: 10.1186/1472-6882-11-29.
6.Arreola R., Quintero-Fabián S., López-Roa R.I., va boshq. Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review // Nutrients. – 2020. – Vol. 12, No. 3. – Article 872.
7.Review: Antimicrobial properties of allicin used alone or in combination with other medications // Frontiers in Microbiology. – 2020. – DOI
8.Gholamnezhad Z., va boshq. Preclinical and clinical effects of Nigella sativa and its constituent, thymoquinone: A review // Journal of Ethnopharmacology. – 2016.
9.Clinical and Laboratory Standards Institute. M07: Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. – 12th ed. – Wayne, PA: CLSI, 2024.
10.Balouiri M., Sadiki M., Ibnsouda S.K. Methods for in vitro evaluating antimicrobial activity: A review // Journal of Pharmaceutical Analysis. – 2016. – Vol. 6, No. 2. – P. 71–79.
