DETERMINATION OF QUANTITATIVE PROPERTIES OF CAMEL MILK PROTEINS BY THE ACTION OF PROTEOLYTIC ENZYMES

Authors

  • M Yuldasheva Tashkent pharmaceutical institute
  • U Ishimov Tashkent pharmaceutical institute

DOI:

https://doi.org/10.5281/zenodo.18401618

Keywords:

camel milk, biologically active peptides, pepsin, papain, bromelain, Lowry method, electrophoresis.

Abstract

In this scientific article, biologically active peptide fractions formed from proteins in camel milk using proteolytic enzymes were analyzed. In the research process, camel milk proteins were separated using physicochemical methods and hydrolyzed using pepsin, papain and bromelain enzymes. The amount of proteins in the obtained hydrolysates and extracts was determined using the Lowry method, and the molecular weights of peptide and protein fractions were estimated by electrophoretic analysis. According to the results, it was determined that the protein content in the sample hydrolyzed with the pepsin enzyme was highly preserved and that biologically active peptides with low molecular weights of up to 20 kDa were present.

References

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ Protein measurement with the Folin phenol reagent . Journal of Biological Chemistry , 1951 .

Walstra P., Wouters JTM, Geurts TJ Dairy Science and Technology. CRC Press, Boca Raton, 2006.

Fox PF, McSweeney PLH Advanced Dairy Chemistry. Volume 1: Proteins. Springer, New York, 2015.

El-Agamy E.I. Bioactive components in camel milk. Food Chemistry, 2009.

Konuspayeva G., Faye B., Loiseau G. The composition of camel milk: A meta-analysis. Journal of Food Composition and Analysis, 2009.

Meisel H., FitzGerald R.J. Biofunctional peptides from milk proteins: Mineral binding and cytomodulatory effects.Current Pharmaceutical Design, 2003.

Nielsen P.M., Petersen D., Dambmann C. Improved method for determining food protein degree of hydrolysis. Journal of Food Science, 2001.

Damodaran S., Parkin K.L., Fennema O.R. Fennema’s Food Chemistry. CRC Press, 2017.

Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970.

Pihlanto A. Antioxidative peptides derived from milk proteins. International Dairy Journal, 2006.

Abd El-Salam M.H., El-Shibiny S. Bioactive peptides of buffalo, camel, goat, sheep, mare, and yak milks and milk products. Food Reviews International, 2013.

Murray R.K., Bender D.A., Botham K.M. Harper’s Illustrated Biochemistry.

McGraw-Hill Education, 2018.

Downloads

Published

2026-01-27

How to Cite

Yuldasheva, M., & Ishimov, U. (2026). DETERMINATION OF QUANTITATIVE PROPERTIES OF CAMEL MILK PROTEINS BY THE ACTION OF PROTEOLYTIC ENZYMES. Academic Research in Modern Science, 5(3), 110-113. https://doi.org/10.5281/zenodo.18401618